TRNA dimethylallyltransferase
| TRNA dimethylallyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.5.1.75 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
TRNA dimethylallyltransferase (EC 2.5.1.75, tRNA prenyltransferase, MiaA, transfer ribonucleate isopentenyltransferase, Delta2-isopentenyl pyrophosphate:tRNA-Delta2-isopentenyl transferase, Delta2-isopentenyl pyrophosphate:transfer ribonucleic acid Delta2-isopentenyltransferase) is an enzyme with systematic name dimethylallyl-diphosphate: tRNA dimethylallyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction
- dimethylallyl diphosphate + tRNA diphosphate + tRNA containing 6-dimethylallyladenosine
Formerly known as tRNA isopentenyltransferase (EC 2.5.1.8), but it is now known that dimethylallyl diphosphate, rather than isopentenyl diphosphate, is the substrate.
Structural studies
[edit]As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2QGN.
References
[edit]- ^ Leung HC, Chen Y, Winkler ME (May 1997). "Regulation of substrate recognition by the MiaA tRNA prenyltransferase modification enzyme of Escherichia coli K-12". The Journal of Biological Chemistry. 272 (20): 13073–83. doi:10.1074/jbc.272.20.13073. PMID 9148919.
- ^ Soderberg T, Poulter CD (May 2000). "Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: essential elements for recognition of tRNA substrates within the anticodon stem-loop". Biochemistry. 39 (21): 6546–53. doi:10.1021/bi992775u. PMID 10828971.
- ^ Moore JA, Mathis JR, Poulter CD (June 2000). "Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: pre-steady-state kinetic studies". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1479 (1–2): 166–74. doi:10.1016/S0167-4838(00)00031-5. PMID 11004538.
Literature
[edit]- Kline LK, Fittler F, Hall RH (1969). "N6-(Δ2-isopentenyl)adenosine. Biosynthesis in transfer ribonucleic acid in vitro". Biochemistry. 8 (11): 4361–4371. doi:10.1021/bi00839a021. PMID 4311031.
- Rosenbaum N, Gefter ML (1972). "Δ2-Isopentenylpyrophosphate: Transfer Ribonucleic Acid Δ2-Isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme". J. Biol. Chem. 247 (18): 5675–5680. doi:10.1016/S0021-9258(19)44812-6. PMID 4341485.
External links
[edit]- TRNA+dimethylallyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
